The Bartholin's gland is located at the entrance to the vagina, and it secretes mucus, providing vulval and vaginal lubrication. A Bartholin's cyst occurs when the gland becomes blocked, and this can Women have two Bartholin's glands, located on each side of the entrance to the vagina. The glands make a fluid that helps lubricate the vulva (external female genitalia) and vagina. Sometimes, a The Normal Vulva and Vagina Springer Nature Link Genitals Sex and Facts